Despite the benefits of mushroom consumption, the bioaccumulation of trace metals in mushrooms can endanger consumer’s health. The consumption of mushrooms in South Eastern Nigeria has received less attention. Seven edible mushroom species (Auricular auricular judae, Lentinus squarrosulus, Pleurotus pulmonarius, Pleurotus ostreatus, Termitomyces microcarpus, Termitomyces robusta and Volvariella volvacea) and their respective substrate samples, collected from Ohiya mechanic village in Abia central senatorial zone of the South-eastern Nigeria were investigated for the presence of five heavy metals (Cd, Pb, Fe, Hg, As). Apart from this, the heavy metals associated health risks were also analyzed for the consumers by evaluating estimated weekly intake (EWI), non-carcinogenic and carcinogenic health risks to ALC and HLC. A total of 21 fruiting bodies (3 specimens for each species) and the corresponding substrates (n = 21) were collected randomly. The results showed that all the mushrooms showed almost same bioconcentration factors, although their metal contents varied among themselves. The magnitude of heavy metals in mushroom fruit bodies tends to increase with an increase of the metals in the substrate. Elevated concentration of heavy metals was observed in those mushroom species collected from highly contaminated substrate. Amomg the heavy metals, iron showed highest mean concentration in the mushroom fruit bodies (85.86±2.39 mg/kg) while least value was recorded for mercury (0.018 mg/kg). Overall, the trace metal contents showed a descending pattern of Fe > Pb > Cd> As > Hg both for mushrooms and their respective substrates. The estimated weekly intake (EWI) values of heavy metals were all lower than provisional tolerable weekly intakes (PTWIs) except for Fe. The highest weekly intake was calculated for Fe, while the lowest value was recorded for Hg. The hazard index (HI), ranged from 1.36-3.59, revealed possibility of adverse health effects on the long term due to the excessive consumption of contaminated wild mushrooms.