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Tuesday, 16 October 2012

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SPAWNING STRATEGY FOR THE CULTIVATION OF PLEUROTUS EOUS (BERKELEY) SACCARDO

Mushroom is unique horticultural crop. In contrast to cultivation of higher plants, which started in pre-historical times, the culture of fungi is relatively recent innovation. Historically, mushroom were gathered from the wild for consumption and for medicinal use. The first commercial cultivation of edible mushrooms was developed in France in the 18th century since then it has traveled far a head. The pink coloured oyster mushroom, Pleurotus eous is one of the edible mushrooms that can be cultivated on a wide range of agro-waste using different methods of spawning. It is relatively less documented species, therefore, there is a vast scope of study on its cultivation.   



Cultivation methodology

 Cultivation of Pleurotus eous is easy with relatively less complicated procedures than other mushroom speciesWheat straw is used as a growing medium for this purpose. It is washed in fresh water and then pasteurized in the solution of Formaldehyde (500ppm) and Bavistin (75ppm) for 18 hours. Under aseptic conditions, it is  inoculated with planting spawn of P. eous (@ 5% w/w on dry wt basis)following the methods given below. 
a)Top spawning: In this method, spawn is planted just above the surface of substrate. It is then covered with a thin layer of substrate.
b)Thorough spawning: It is achieved by thoroughly mixing of spawn with the substrate while filling the bags.
c)Layer spawning: The spawn is planted in the substrate in multilayered (3) manner. 
Spawned substrates can be filled in perforated polythene bags and incubated in cultivation room at 22-30°C temperature for mycelial growthOnce the mycelia has completely colonized the wheat straw substrate, the polythene covering is turned off and relative humidity is maintained 85-95 per cent with the help of humidifier. Approximately 2 to 4 days after opening the bags, mushroom primordia will begin to appear. To harvest the mushrooms, they should be grasped by the stalk and gently twisted and pulled. After harvesting lower portion of the stalk with adhering debris should be cutoff using a knife.


Effect of spawning technique

  The methods of spawning have prime importance in mushroom cultivation. The proper and homogeneous mixing of spawn enhances growth of mushroom mycelium through the substrate and resulted early fructification. Thorough spawning fulfills these criteria and hence prove better over other spawning techniques (Table). 


Effect of various spawning methods on yield performance of Pleurotus eous
  The yield of mushroom is greatly influenced on cultural conditions e.g. quality and quantity of spawn, amount and nature of substrate used, cultivation method (Bag culture), environmental conditions and number of harvest(3)
(A) Thorough spawning
(B) Layer spawning
(C) Top spawning
                       Effect of spawning techniques on cropping of P. eous
                         

If the similar cultural conditions are provided, spawning techniques does't show significant difference in mushroom yield (Table). However, the overall scenario can be changed if harvesting period takes priority over number of harvest (Figures.1&2).(See Original)
      Figure1. Yield of P. eous in different flushes
                                                             Figure2. Yield of P. eous on 22th day of harvest     

Cite this as: Siddhant, Swapnil Yadav and C.S. Singh (2013). Spawn and Spawning Strategies for the Cultivation of Pleurotus eous (Berkeley) Saccardo. International Journal of Pharmaceutical and chemical sciences. 2(3):1494-1500 

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